gap43 (Novus Biologicals)
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gap43
Gap43, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gap+43/GAP-43+Antibody/pm41885803-142-63-65
Average 94 stars, based on 1 article reviews
Gap43, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gap+43/GAP-43+Antibody/pm41885803-142-63-65
Average 94 stars, based on 1 article reviews
gap43 - by Bioz Stars,
2026-10
94/100 stars
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Western Blot:Article Title: Inhibiting acute, axonal DLK palmitoylation is neuroprotective and avoids deleterious effects of cell-wide DLK inhibition Article Snippet: The following primary antibodies, raised in the indicated species, were purchased from Cell Signaling Technology: phospho–c-Jun (Ser63) (rabbit, #91952, used at 1:500 dilution for western blot (WB), 1:100 for immunocytochemistry and immunohistochemistry (ICC, IHC); phospho–c-Jun (Ser73) (rabbit, #3270, used at 1:500 for WB); myc (rabbit, #2278 used at 1:100 for ICC); alpha-tubulin (mouse, #3873, used at 1:2000 for WB); phospho-MKK4 (rabbit, #4514, used at 1:1000 for WB); pan-MKK4 (rabbit, #9152 used at 1:1000 for WB); Lamin A/C (mouse, #4777 used at 1:400 for WB), phospho-Akt (Thr308) rabbit #9275, used at 1:250 for WB), Akt (rabbit, #4691, used at 1:1000 for WB), pan-ERK (rabbit, #4635, used at 1:1000 for western blot), phospho-ERK1/2 (mouse #9106, used at 1:250 for WB),. .. Additional antibodies were from the following indicated suppliers: anti-NGF (sheep, CedarLane, #CLMCNET-031, used at 1:40 dilution for live cell assays); DLK/MAP3K12 (rabbit, Genetex, #GTX124127, used at 1:5000 for WB); β3 tubulin (mouse, BioLegend, TUJ1, #MMS-435P, used at 1:1000 for ICC), NF-200 (mouse, MilliporeSigma #NO-142, used at 1:1000 for ICC), VAMP2 (mouse, Synaptic Systems, #104211SY, used at 1:1000 for ICC), Brn3a (mouse, Millipore Sigma, # MAB1585, used at 1:100 for IHC), Article Title: Inhibiting acute, axonal DLK palmitoylation is neuroprotective and avoids deleterious effects of cell-wide DLK inhibition. Article Snippet: The following primary antibodies, raised in the indicated species, were purchased from Cell Signaling Technology: phospho–c-Jun (Ser63) (rabbit, #91952, used at 1:500 dilution for western blot (WB), 1:100 for immunocytochemistry and immunohistochemistry (ICC, IHC); phospho–c-Jun (Ser73) (rabbit, #3270, used at 1:500 for WB); myc (rabbit, #2278 used at 1:100 for ICC); alpha-tubulin (mouse, #3873, used at 1:2000 for WB); phospho-MKK4 (rabbit, #4514, used at 1:1000 for WB); pan-MKK4 (rabbit, #9152 used at 1:1000 for WB); Lamin A/C (mouse, #4777 used at 1:400 for WB), phospho-Akt (Thr308) rabbit #9275, used at 1:250 for WB), Akt (rabbit, #4691, used at 1:1000 for WB), pan-ERK (rabbit, #4635, used at 1:1000 for western blot), phospho-ERK1/2 (mouse #9106, used at 1:250 for WB),. .. Additional antibodies were from the following indicated suppliers: anti-NGF (sheep, CedarLane, #CLMCNET-031, used at 1:40 dilution for live cell assays); DLK/MAP3K12 (rabbit, Genetex, #GTX124127, used at 1:5000 for WB); β3 tubulin (mouse, BioLegend, TUJ1, #MMS-435P, used at 1:1000 for ICC), NF-200 (mouse, MilliporeSigma #NO-142, used at 1:1000 for ICC), VAMP2 (mouse, Synaptic Systems, #104211SY, used at 1:1000 for ICC), Brn3a (mouse, Millipore Sigma, # MAB1585, used at 1:100 for IHC), Immunocytochemistry:Article Title: Inhibiting acute, axonal DLK palmitoylation is neuroprotective and avoids deleterious effects of cell-wide DLK inhibition Article Snippet: The following primary antibodies, raised in the indicated species, were purchased from Cell Signaling Technology: phospho–c-Jun (Ser63) (rabbit, #91952, used at 1:500 dilution for western blot (WB), 1:100 for immunocytochemistry and immunohistochemistry (ICC, IHC); phospho–c-Jun (Ser73) (rabbit, #3270, used at 1:500 for WB); myc (rabbit, #2278 used at 1:100 for ICC); alpha-tubulin (mouse, #3873, used at 1:2000 for WB); phospho-MKK4 (rabbit, #4514, used at 1:1000 for WB); pan-MKK4 (rabbit, #9152 used at 1:1000 for WB); Lamin A/C (mouse, #4777 used at 1:400 for WB), phospho-Akt (Thr308) rabbit #9275, used at 1:250 for WB), Akt (rabbit, #4691, used at 1:1000 for WB), pan-ERK (rabbit, #4635, used at 1:1000 for western blot), phospho-ERK1/2 (mouse #9106, used at 1:250 for WB),. .. Additional antibodies were from the following indicated suppliers: anti-NGF (sheep, CedarLane, #CLMCNET-031, used at 1:40 dilution for live cell assays); DLK/MAP3K12 (rabbit, Genetex, #GTX124127, used at 1:5000 for WB); β3 tubulin (mouse, BioLegend, TUJ1, #MMS-435P, used at 1:1000 for ICC), NF-200 (mouse, MilliporeSigma #NO-142, used at 1:1000 for ICC), VAMP2 (mouse, Synaptic Systems, #104211SY, used at 1:1000 for ICC), Brn3a (mouse, Millipore Sigma, # MAB1585, used at 1:100 for IHC), Article Title: Inhibiting acute, axonal DLK palmitoylation is neuroprotective and avoids deleterious effects of cell-wide DLK inhibition. Article Snippet: The following primary antibodies, raised in the indicated species, were purchased from Cell Signaling Technology: phospho–c-Jun (Ser63) (rabbit, #91952, used at 1:500 dilution for western blot (WB), 1:100 for immunocytochemistry and immunohistochemistry (ICC, IHC); phospho–c-Jun (Ser73) (rabbit, #3270, used at 1:500 for WB); myc (rabbit, #2278 used at 1:100 for ICC); alpha-tubulin (mouse, #3873, used at 1:2000 for WB); phospho-MKK4 (rabbit, #4514, used at 1:1000 for WB); pan-MKK4 (rabbit, #9152 used at 1:1000 for WB); Lamin A/C (mouse, #4777 used at 1:400 for WB), phospho-Akt (Thr308) rabbit #9275, used at 1:250 for WB), Akt (rabbit, #4691, used at 1:1000 for WB), pan-ERK (rabbit, #4635, used at 1:1000 for western blot), phospho-ERK1/2 (mouse #9106, used at 1:250 for WB),. .. Additional antibodies were from the following indicated suppliers: anti-NGF (sheep, CedarLane, #CLMCNET-031, used at 1:40 dilution for live cell assays); DLK/MAP3K12 (rabbit, Genetex, #GTX124127, used at 1:5000 for WB); β3 tubulin (mouse, BioLegend, TUJ1, #MMS-435P, used at 1:1000 for ICC), NF-200 (mouse, MilliporeSigma #NO-142, used at 1:1000 for ICC), VAMP2 (mouse, Synaptic Systems, #104211SY, used at 1:1000 for ICC), Brn3a (mouse, Millipore Sigma, # MAB1585, used at 1:100 for IHC), Immunohistochemistry:Article Title: Inhibiting acute, axonal DLK palmitoylation is neuroprotective and avoids deleterious effects of cell-wide DLK inhibition Article Snippet: The following primary antibodies, raised in the indicated species, were purchased from Cell Signaling Technology: phospho–c-Jun (Ser63) (rabbit, #91952, used at 1:500 dilution for western blot (WB), 1:100 for immunocytochemistry and immunohistochemistry (ICC, IHC); phospho–c-Jun (Ser73) (rabbit, #3270, used at 1:500 for WB); myc (rabbit, #2278 used at 1:100 for ICC); alpha-tubulin (mouse, #3873, used at 1:2000 for WB); phospho-MKK4 (rabbit, #4514, used at 1:1000 for WB); pan-MKK4 (rabbit, #9152 used at 1:1000 for WB); Lamin A/C (mouse, #4777 used at 1:400 for WB), phospho-Akt (Thr308) rabbit #9275, used at 1:250 for WB), Akt (rabbit, #4691, used at 1:1000 for WB), pan-ERK (rabbit, #4635, used at 1:1000 for western blot), phospho-ERK1/2 (mouse #9106, used at 1:250 for WB),. .. Additional antibodies were from the following indicated suppliers: anti-NGF (sheep, CedarLane, #CLMCNET-031, used at 1:40 dilution for live cell assays); DLK/MAP3K12 (rabbit, Genetex, #GTX124127, used at 1:5000 for WB); β3 tubulin (mouse, BioLegend, TUJ1, #MMS-435P, used at 1:1000 for ICC), NF-200 (mouse, MilliporeSigma #NO-142, used at 1:1000 for ICC), VAMP2 (mouse, Synaptic Systems, #104211SY, used at 1:1000 for ICC), Brn3a (mouse, Millipore Sigma, # MAB1585, used at 1:100 for IHC), Article Title: Inhibiting acute, axonal DLK palmitoylation is neuroprotective and avoids deleterious effects of cell-wide DLK inhibition. Article Snippet: The following primary antibodies, raised in the indicated species, were purchased from Cell Signaling Technology: phospho–c-Jun (Ser63) (rabbit, #91952, used at 1:500 dilution for western blot (WB), 1:100 for immunocytochemistry and immunohistochemistry (ICC, IHC); phospho–c-Jun (Ser73) (rabbit, #3270, used at 1:500 for WB); myc (rabbit, #2278 used at 1:100 for ICC); alpha-tubulin (mouse, #3873, used at 1:2000 for WB); phospho-MKK4 (rabbit, #4514, used at 1:1000 for WB); pan-MKK4 (rabbit, #9152 used at 1:1000 for WB); Lamin A/C (mouse, #4777 used at 1:400 for WB), phospho-Akt (Thr308) rabbit #9275, used at 1:250 for WB), Akt (rabbit, #4691, used at 1:1000 for WB), pan-ERK (rabbit, #4635, used at 1:1000 for western blot), phospho-ERK1/2 (mouse #9106, used at 1:250 for WB),. .. Additional antibodies were from the following indicated suppliers: anti-NGF (sheep, CedarLane, #CLMCNET-031, used at 1:40 dilution for live cell assays); DLK/MAP3K12 (rabbit, Genetex, #GTX124127, used at 1:5000 for WB); β3 tubulin (mouse, BioLegend, TUJ1, #MMS-435P, used at 1:1000 for ICC), NF-200 (mouse, MilliporeSigma #NO-142, used at 1:1000 for ICC), VAMP2 (mouse, Synaptic Systems, #104211SY, used at 1:1000 for ICC), Brn3a (mouse, Millipore Sigma, # MAB1585, used at 1:100 for IHC), Marker:Article Title: Interaction of reactive astrocytes with type I collagen induces astrocytic scar formation through the integrin-N-cadherin pathway after spinal cord injury. Article Snippet: The sections were subsequently stained with primary antibodies in blocking solution at 4 °C overnight and were then incubated with Alexa Fluor–conjugated secondary antibodies (1:200; Invitrogen) and Hoechst 33258. .. The following primary antibodies were used: GFAP (1:200; rabbit; Dako, Z0334), GFAP (1:200; rat; Life Technologies, 130300), nestin (1:200; rat; a gift from T. Tachibana, Osaka City University), ß-catenin (1:200; mouse; Millipore, 05-665), N-cadherin (1:200; mouse; BD Transduction Laboratories, 610921), Sox9 (1:200; rabbit; Abcam, ab26414), COL1A1 (1:200; mouse; Sigma-Aldrich, SAB1402151), Incubation:Article Title: Optogenetic Neuronal Stimulation Promotes Functional Recovery After Spinal Cord Injury Article Snippet: .. The blots were blocked with 5% milk for 1 h at room temperature and were subsequently incubated overnight at 4°C with the following primary antibodies: other:Article Title: Reverse-turn mimetics and method relating thereto Article Snippet: Transfections were performed using Superfect (Qiagen) according to manufacturer's instructions. Article Title: Environmental stress promotes the persistence of juvenile traits in olfactory neurons as a protective mechanism Article Snippet: A minimum of two cryosections per animal were acquired by confocal microscopy (SP5; Leica). |